Here are the 55 US AI startups that raised $100M or more in 2025
The AI Landscape in the US: A Comprehensive Look at the 55 US AI Startups that Raised $100M or More in 2025
The AI industry has been on a tear in recent years, with a record number of startups raising significant funding rounds in 2025. According to our analysis, 55 US AI startups raised $100 million or more in 2025, a testament to the growing demand for AI solutions across various industries. In this article, we'll take a closer look at these startups, their funding rounds, and what they're doing to revolutionize the AI landscape.
December 2025: A Busy Month for AI Startups
December 2025 was a particularly busy month for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by Mythic, a power-efficient compute company based in Austin, Texas. Mythic raised a $125 million venture round led by DCVC, with participation from SoftBank, NEA, and Linse Capital, among others. This round valued the company at $1.2 billion.
Another notable round was raised by Chai Discovery, a biotech and drug discovery company that builds AI models. Chai Discovery announced a $130 million Series B round co-led by Oak HC/FT and General Catalyst, valuing the company at $1.2 billion.
Generative media platform Fal also announced a significant funding round in December, raising a $140 million Series D round led by Sequoia, valuing the company at over $4.5 billion.
November 2025: AI Startups Continue to Raise Big
November 2025 was another busy month for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by Luma AI, a company that builds models used for photo and video creation. Luma AI raised $900 million in a Series C round led by Humain, valuing the company at $4 billion.
Viral vibe-coding platform Cursor also raised a significant round in November, raising $2.3 billion in a funding round that valued the company at $29.3 billion.
Parallel, a web infrastructure company for AI agents, raised a $100 million Series A round co-led by Index Ventures and Kleiner Perkins.
October 2025: AI Startups Continue to Innovate
October 2025 was a month of innovation for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by Fireworks AI, a platform that allows users to build AI applications using open source models. Fireworks AI raised a $250 million Series C round, valuing the company at $4 billion.
Enterprise AI startup Uniphore also raised a significant round in October, raising a $260 million Series F round, valuing the company at $2.5 billion.
Sesame, a voice AI company, raised a $250 million Series B round co-led by Sequoia and Spark Capital.
September 2025: AI Startups Continue to Raise Funding
September 2025 was a month of significant funding rounds for AI startups. One of the most notable rounds was raised by Cerebras Systems, an AI infrastructure company that raised a $1.1 billion Series G round, valuing the company at $8.1 billion.
Modular, a company that builds AI enterprise software, raised a $250 million funding round, valuing the company at $1.8 billion.
Upscale AI, an AI infrastructure startup, raised a $100 million seed round, valuing the company at $580 million.
August 2025: AI Startups Continue to Innovate
August 2025 was a month of innovation for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by EliseAI, a healthcare and housing automation platform that raised $250 million in a Series E round, valuing the company at $2.2 billion.
Decart, an AI research lab, raised $100 million at a $3.1 billion valuation.
July 2025: AI Startups Continue to Raise Funding
July 2025 was a month of significant funding rounds for AI startups. One of the most notable rounds was raised by Ambience Healthcare, a company that builds an AI healthcare operating system, which raised a $243 million Series C round, valuing the company at $1 billion.
Reka AI, an AI research lab, raised $110 million in a Series B round, valuing the company at $1 billion.
June 2025: AI Startups Continue to Innovate
June 2025 was a month of innovation for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by Abridge, a healthcare AI unicorn that raised a $300 million Series E round, valuing the company at $5.3 billion.
Harvey, a company that builds AI tools for the legal industry, raised a $300 million Series E round, valuing the company at $5 billion.
May 2025: AI Startups Continue to Raise Funding
May 2025 was a month of significant funding rounds for AI startups. One of the most notable rounds was raised by Snorkel AI, a company that builds AI data labeling software, which raised a $100 million Series D round, valuing the company at $1.3 billion.
LMArena, a community-driven benchmarking tool for AI models, raised a $100 million seed round, valuing the company at $600 million.
April 2025: AI Startups Continue to Innovate
April 2025 was a month of innovation for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by SandboxAQ, a company that builds AI models, which raised a $450 million Series E round, valuing the company at $5.7 billion.
Runway, a company that creates AI models for media production, raised a $308 million Series D round, valuing the company at $3 billion.
March 2025: AI Startups Continue to Raise Funding
March 2025 was a month of significant funding rounds for AI startups. One of the most notable rounds was raised by OpenAI, a company that builds AI models, which raised a record-breaking $40 billion funding round, valuing the company at $300 billion.
Nexthop AI, an AI infrastructure company, raised a $110 million Series A round, valuing the company at $580 million.
February 2025: AI Startups Continue to Innovate
February 2025 was a month of innovation for AI startups, with several notable funding rounds announced. One of the most significant rounds was raised by Together AI, a company that builds open source generative AI and AI model development infrastructure, which raised a $305 million Series B round, valuing the company at $3.3 billion.
Lambda, an AI infrastructure company, raised a $480 million Series D round, valuing the company at $2.5 billion.
January 2025: AI Startups Continue to Raise Funding
January 2025 was a month of significant funding rounds for AI startups. One of the most notable rounds was raised by ElevenLabs, a company that builds synthetic voice technology, which raised a $180 million Series C round, valuing the company at $3 billion.
Hippocratic AI, a company that builds large language models for the healthcare industry, raised a $141 million Series B round, valuing the company at $1.6 billion.
Conclusion
The AI landscape in the US is rapidly evolving, with a record number of startups raising significant funding rounds in 2025. From power-efficient compute to AI models for media production, these startups are pushing the boundaries of what is possible with AI. As the industry continues to grow and mature, we can expect to see even more innovative solutions and applications emerge.
